terminator sequence การใช้

• Owing to its greater expediency and speed, dye-terminator sequencing is now the mainstay in automated sequencing.
• This process is known as rho-independent or intrinsic termination, and the sequences involved are called terminator sequences.
• In dye-terminator sequencing, each of the four dideoxynucleotide chain terminators is labelled with fluorescent dyes, each of which emit light at different wavelengths.
• The cyano-1 RNA motif family is essentially a synonym for Yfr2, although Cyano-1 does not include the terminator sequence and Yfr2 does.
• The dye-terminator sequencing method, along with automated high-throughput DNA sequence analyzers, is now being used for the vast majority of sequencing projects.
• When SAM is unbound, the anti-terminator sequence sequesters the terminator sequence so the terminator is unable to form, allowing the polymerase read-through the downstream gene.
• When SAM is unbound, the anti-terminator sequence sequesters the terminator sequence so the terminator is unable to form, allowing the polymerase read-through the downstream gene.
• "Dye-terminator sequencing " utilizes labelling of the chain terminator ddNTPs, which permits sequencing in a single reaction, rather than four reactions as in the labelled-primer method.
• Those meant to transform prokaryotic genomes generally have the gene or genes of interest, at least one terminator sequence, and a reporter gene; which is a gene used to enable detection or removal of those cells which didn't integrate the construct into their DNA.
• Constructs meant for integration into a eukaryotic nucleus follow a similar pattern except that : the construct contains no border sequences because the sequence rearrangement that prokaryotic constructs rely on rarely occurs in eukaryotes; and each gene contained within the construct must be expressed by its own copy of a promoter and terminator sequence.
• The initial binding site for Rho is an extended ( ~ 70 nucleotides, sometimes 80 100 nucleotides ) single-stranded region, rich in cytosine and poor in guanine, called the "'r "'ho "'ut "'ilisation site ( "'rut "'), in the RNA being synthesised, upstream of the actual terminator sequence.